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Instrument Operating Procedure in our lab

Microbiological Laboratory Section

Instruments Name
Water-Bath
Incubator-01
Incubator-02
Incubator-03
Vortex
Microscope
Colony Counter

WATER-BATH

Lab ID -PL/DWB/02


Pour distilled water into the water path upto atleast 5 cm above the heating cell in immersed condition.
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Switch on the hot water bath & set the temperature to 45 ± 1 ℃.
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Constant temperature is reached with thermostat control.
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After the work is over, switch off the equipment.

Precaution

  • Ensure the water level is adequate before switching on the water bath.
  • Use distilled water to prevent scale formation.
  • Handle hot vessels with tongs or heat-resistant gloves.
  • Avoid spilling water on electrical parts.
  • Repeat the calibration process or inform the technical manager if variation exceed the ±5% of expected value.

INCUBATOR 01

Lab ID -PL/INC/01

Switch on the Incubator
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Ensure incubator is disinfected (70% alcohol) and dry
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Switch on the thermostat & set the required temperature 37±1℃ (E.Coli & Coliform in food & water sample)
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Allow incubator to preheat and stabilize (15–30 min)
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Place culture media/plates inside incubator with proper labelling
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After incubation, remove cultures carefully.Switch OFF incubator (if not in continuous use)

Precaution

  • Avoid frequent opening of the door to maintain constant temperature.
  • Keep the incubator clean and dry to prevent contamination.
  • Do not overload the incubator.
  • Regularly calibrate temperature.Inform the technical manager if variation exceed the ±5% of expected value.Keep a temperature log record.

INCUBATOR 02

Lab ID -PL/INC/02

Switch on the Incubator
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Ensure incubator is disinfected (70% alcohol) and dry
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Switch on the thermostat & set the required temperature 25±1℃ for Yeast & Mold testing
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Allow incubator to preheat and stabilize (15–30 min)
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Place culture media/plates inside incubator with proper labelling
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After incubation, remove cultures carefully.Switch OFF incubator (if not in continuous use)

Precaution

  • Avoid frequent opening of the door to maintain constant temperature.
  • Keep the incubator clean and dry to prevent contamination.
  • Do not overload the incubator.
  • Regularly calibrate temperature.Inform the technical manager if variation exceed the ±5% of expected value.Keep a temperature log record.

INCUBATOR 03

Lab ID -PL/INC/03

Switch on the Incubator
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Ensure incubator is disinfected (70% alcohol) and dry
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Switch on the thermostat & set the required temperature 30±1℃ (Total plate count Test in food sample)
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Allow incubator to preheat and stabilize (15–30 min)
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Place culture media/plates inside incubator with proper labelling
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After incubation, remove cultures carefully.Switch OFF incubator (if not in continuous use)

Precaution

  • Avoid frequent opening of the door to maintain constant temperature.
  • Keep the incubator clean and dry to prevent contamination.
  • Do not overload the incubator.
  • Regularly calibrate temperature.Inform the technical manager if variation exceed the ±5% of expected value.Keep a temperature log record.

VORTEX

Lab ID -PL/MB/MV/01

Switch on the Instrument
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Ensure rubber cup/head is clean and properly fixed
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Place the mixer on a stable, flat surface
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Adjust speed control knob to required speed
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Hold the test tube firmly (with cap closed)
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Allow mixing until solution becomes uniform
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Remove tube from mixer
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Switch OFF the instrument
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Clean the rubber head if any spillage occurred

Precautions

  • Always cap tubes tightly before mixing.
  • Avoid excessive speed for fragile tubes.
  • Do not spill infectious materials.
  • Disinfect after use if contaminated.

MICROSCOPE

Lab ID -PL/MB/MS/01

Place microscope on a clean, stable table & Check cleanliness of lenses
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Switch ON light source
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Place prepared slide on stage.Secure slide with stage clips
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Select low power objective (10X)
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Adjust coarse focus knob to obtain image,fine focus knob for clarity,diaphragm and light intensity
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Switch to high power objective (40X) if needed.Use fine adjustment only for focusing
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For oil immersion (100X) Place a drop of immersion oil on slide
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Rotate to 100X objective.Focus using fine adjustment only
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Observe and record findings.Remove slide carefully,Clean oil from objective.
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Switch OFF light.Cover microscope properly.

Precaution

  • Always start with low power objective.
  • Never use coarse knob on high power or oil immersion.
  • Do not touch lenses with fingers.
  • Handle microscope with both hands.

COLONY COUNTER

Lab ID -PL/MB/CC/01


Clean the counting platform with disinfectant
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Switch ON the colony counter.Adjust light intensity and magnifier position
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Place Petri plate (inverted) on counting grid
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Mark each colony using marker tip probe
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Press counting button for each colony counted
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Ensure each colony is counted only once
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Complete counting of entire plate
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Note final colony count from digital display & record result
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Remove Petri plate carefully
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Switch OFF the instrument
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Clean surface if contaminated

Precaution

  • Count plates with 30–300 colonies (ideal range).
  • Avoid double counting.
  • Handle plates aseptically.
  • Do not press too hard on agar surface.
  • Disinfect after handling contaminated samples.